Cell and Tissue Biotechnology
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- Cell and Tissue Biotechnology
Field of study
• Cell electroporation research: from fundamental research to applications in biotechnology, medicine and veterinary medicine.
• Development of anticancer therapy based on irreversible electroporation and electrochemotherapy (with anticancer drugs or calcium electroporation) separately and in combination with radiotherapy.
• Studies on the transfer of nucleic acids, proteins and other molecules into cells and tissues for genetic modification of cells and vaccination.
• Research and applications of cell sonoporation.
• Controlled inactivation and genetic modification of various bacteria.
• Application of irreversible electroporation to cardiac tissue ablation to suppress atrial fibrillation.
• Development and commercialization of electroporation equipment and tools. A green company UAB Amber Charge was founded https://ambercharge.eu
People
Principal Scientist
Senior Scientists
Scientists
Doctoral Students
Defended dissertaions (over the last 5 years)
2024
Dovilė Uždavinytė. “Investigation of plasma membrane repair and cell death mechanisms after electroporation and application in anticancer therapy”. Natural sciences, Biophysics – N 011. Scientific advisor doc. dr. Ingrida Šatkauskienė
2023
Rūta Palepšienė. “Study of factors affecting electrotransfer of nucleic acids into cells“. Natural sciences, Biophysics – N 011. Scientific advisor Prof. dr. Saulius Šatkauskas
2021
Diana Navickaitė. “Influence of calcium ions on molecular transport through plasma membrane and cell viability after electroporation and sonoporation “. Natural sciences, Biophysics – N 011. Scientific advisor Prof. dr. Saulius Šatkauskas
2020
Sonam Chopra. “Independent and combined intracellular electrotransfer of DNA and small molecules“. Natural Sciences, Biology – N 010. Scientific advisor Prof. dr. Saulius Šatkauskas
2019
Baltramiejus Jakštys. “Investigation of cell membrane permeability, efflux of small intracellular molecules and cell viability after electroporation” Natural sciences, Biophysics – N 011. Scientific advisor Prof. dr. Saulius Šatkauskas
Projects
2024-2027. LMT mokslininkų grupių projektas: Navikų radiojautrumo ir imuninio atsako stiprinimas pasitelkiant multimodalines gydymo strategijas. Vykdytojai Sutarties Nr. MIP-129/24. LSMU ir VDU. VDU pusės vadovas dr. P. Ruzgys.
2023-2024. LMT projektas: „Belaidžio elektroporatoriaus komercializavimas“ Sutarties Nr. S-TPP-23-10. Vadovas dr. S. Šatkauskas.
2023-2026. LMT mokslininkų grupių projektas: Nauja auglių pašalinimo technologija naudojant stipraus magnetinio lauko impulsų sekas valdomai vaistų ir DNR pernašai. Vykdytojai Sutarties Nr. MIP-124/23. VGTU ir VDU. VDU pusės vadovas dr. P. Ruzgys.
2022-2024. LMT mokslininkų stažuotojų projektas: Selektyvi gramteigiamų ir gramneigiamų bakterijų augimo greičio kontrolė, siekiant pagerinti bioprodukcijos efektyvumą naudojant elektrinius laukus. Vadovas dr. S. Šatkauskas.
2021-2022. Mokslo ir inovacijų agentūros finansuojamas projektas Nukleorūgščių pernašos į ląsteles elektroporatoriaus prototipas Nr. 01.2.1-MITA-T-852-02-0250. Vadovas dr. S. Šatkauskas.
2021-2022. Mokslo ir inovacijų agentūros finansuojamas projektas 01.2.2-MITA-K-702 MTEP rezultatų komercinimo ir tarptautiškumo skatinimas Nr. 01.2.2-MITA-K-702-11-0032. Vadovas dr. S. Šatkauskas.
2019-2020. Baltic-German University Liaison Office project. Establishing of electroporation for parallelized genome-scale functional analysis. Lietuvos pusės koordinatorius S. Šatkauskas
2017-2022. LMT projektas: „Bioaktyvių medžiagų pernaša į ląsteles ir audinius taikant elektroporaciją ir sonoporaciją tikslinei ir sisteminei navikų terapijai“ Nr. DOTSUT 09.3.3-LMT-K-712-01-0188. Vadovas dr. S. Šatkauskas.
2014-2015. LMT projektas „Elektroporacijos tyrimai augalinės žaliavos bioaktyvių komponentų masės pernašos intensyvinimui“ pagal nacionalinę mokslo programą “Sveikas ir saugus maistas”. Vadovas dr. S. Šatkauskas.
2014-2015. Lietuvos-Šveicarijos pagal Sciex programą projektas “Electrospray: A novel approach for targeted drug and gene delivery to lung”. Lietuvos pusės vadovas dr. S. Šatkauskas.
2013-2015. LMT projektas: „Mikroburbulų stabilios ir inercinės kavitacijos parametrų įtakos sonoporacijos efektyvumui tyrimas“. MIP-034/2013. Vadovas dr. M. Venslauskas.
2013-2014. LMT mokslininkų stažuotojų projektas „ Elektroporacijos tyrimai ir taikymas biologiškai aktyvių medžiagų ekstrakcijai iš augalinės žaliavos“. Vadovas dr. S. Šatkauskas.
2011-2013. Tarptautinis trišalis Lietuvos-Latvijos-Taivanio projektas „Ląstelių ektroporacijos ir sonoporacijos tyrimai efektyviai vaistų pernašai ir navikų gydymui“. Sutarties nr. Nr. TAP-03/2011. Lietuvos pusės vadovas dr. S. Šatkauskas.
2011-2012. LMT mokslininkų stažuotojų projektas “Vaistus bei genus nešančių mikroburbulų gamyba ir jų savybių tyrimai sonoporacijoje”. Vadovas dr. S. Šatkauskas.
2010-2011. LMT projektas: „Priešvėžinių vaistų pernašos efektyvumo į ląsteles ir navikus, taikant elektro-sonoporaciją tyrimas“. Sutartis MIP-119/2010. Vadovas dr. S. Šatkauskas.
2009-2010. Tarptautinis dvišalių mainų projektas pagal Žilibero programą “Genų ir vaistų pernaša į ląsteles ir audinius naudojant elektrosonoporaciją” Sutarties nr. Nr. 31V-130/2009 (TAP-18/2010). Lietuvos pusės vadovas dr. S. Šatkauskas.
2009-2010. LMT mokslininkų stažuotojų projektas “Genų ir vaistų pernaša į ląsteles naudojant elektroporaciją ir sonoporaciją” Sutarties nr. MOS14/2010 (S-7/2009). Vadovas dr. S. Šatkauskas.
2008-2010. LMT mokslininkų stažuotojų projektas “Semaforinų funkcinis ryšys su matrikso metaloproteinazėmis vystantis nervų sistemai” Sutarties nr. MOS-7/2010 (S-14/2008). Vadovas dr. S. Šatkauskas.
2003-2005. Prioritetinių Lietuvos mokslinių tyrimų ir eksperimentinės plėtros krypčių programa, “Funkcinės nanostruktūros ir molekuliniai mechanizmai” Reg. Nr. C-03046. VDU koordinatorius dr. M. Venslauskas.
1999–2000. VMS fondo mokslinių grupių projektas: “Navikinių ląstelių nekrozės ir apoptozės tyrimai po navikų elektrochemoterapijos”. Vadovas dr. S. Šatkauskas.
Publications
2024
Visockis M, Ruzgys P, Gelažunaitė S, Vykertas S, Šatkauskas S. Detection of Gram-positive and Gram-negative bacteria membrane permeabilization induced by pulsed electric field using electrochemical admittance spectroscopy. Bioelectrochemistry. 2024 Oct 11;161:108835. https://doi.org/10.1016/j.bioelechem.2024.108835
Vadeikienė R, Jakštys B, Laukaitienė D, Šatkauskas S, Juozaitytė E, Ugenskienė R. The Role of Mutated Calreticulin in the Pathogenesis of BCR-ABL1-Negative Myeloproliferative Neoplasms. Int J Mol Sci. 2024 Sep 12;25(18):9873. PMID: 39337361 Free PMC article. https://doi.org/10.3390/ijms25189873
Urbanskas E, Jakštys B, Venckus J, Malakauskaitė P, Šatkauskienė I, Morkvėnaitė-Vilkončienė I, Šatkauskas S. Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer. Pharmaceuticals (Basel). 2024 Jun 23;17(7):825. PMID: 39065676 Free PMC article. https://doi.org/10.3390/ph17070825
Palepšienė R, Muralidharan A, Maciulevičius M, Ruzgys P, Chopra S, Boukany PE, Šatkauskas S. New insights into the mechanism of electrotransfer of small nucleic acids. Bioelectrochemistry. 2024 Apr 4;158:108696. https://doi.org/10.1016/j.bioelechem.2024.108676
Maciulevičius M, Palepšienė R, Vykertas S, Raišutis R, Rafanavičius A, Krilavičius T, Šatkauskas S. The comparison of the dynamics of Ca2+ and bleomycin intracellular delivery after cell sonoporation and electroporation in vitro. Bioelectrochemistry. 2024 Apr 14;158:108708. https://doi.org/10.1016/j.bioelechem.2024.108708
2023
Maciulevičius M, Raišutis R, Jakštys B, Svilainis L, Chaziachmetovas A, Šatkauskas S. The Assessment of Calcium and Bleomycin Cytotoxic Efficiency in Relation to Cavitation Dosimetry. Pharmaceutics. 2023 May 11;15(5):1463. https://doi.org/10.3390/pharmaceutics15051463
Palepšienė R. Maciulevičius M. Ruzgys P., Jakštys B., and Šatkauskas S. The Influence of Calcium Ions on the Electrotransfer Efficiency of Plasmid DNA and Cell Viability. Appl. Sci. 2023, 13(3), 1983. https://doi.org/10.3390/app13031983
2022
Vadeikienė R, Jakštys B, Ugenskienė R, Šatkauskas S, Juozaitytė E. Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model. Biomedicines. 2022 Oct 24;10(11):2687. PMID: 36359207. https://doi.org/10.3390/biomedicines10112687
Ruzgys P, Navickaitė D, Palepšienė R, Uždavinytė D, Barauskaitė N, Novickij V, Girkontaitė I, Šitkauskienė B, Šatkauskas S. Induction of Bystander and Abscopal Effects after Electroporation-Based Treatments. Cancers (Basel). 2022 Aug 2;14(15):3770. (IF = 6.575). https://doi.org/10.3390/cancers14153770
Maciulevičius M, Tamošiūnas M, Jurkonis R, Šatkauskas S. Dosimetric assessment of antitumor treatment by enhanced bleomycin delivery via electroporation and sonoporation. Bioelectrochemistry. 2022. doi: 10.1016/j.bioelechem.2022. (IF = 5.760).
Maciulevičius M., Tamošiūnas M., Navickaitė D., Šatkauskas S., Mindaugas Venslauskas SM. Free- and liposomal- doxorubicin delivery via microbubble inertial cavitation. Journal of Drug Delivery Science and Technology. 2022. 7. (IF = 3.981). https://doi.org/10.1016/j.jddst.2022.103386.
2021
Ruzgys P, Barauskaitė N, Novickij V, Novickij J, Šatkauskas S. The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation. Molecules. 2021 Oct 2;26(19):6001. (IF = 4.412). https://doi.org/10.3390/molecules26196001
Šalaševičius A., Uždavinytė D., Visockis M., Ruzgys P., Šatkauskas S. Effect of Pulsed Electric Field (PEF) on Bacterial Viability and Whey Protein in the Processing of Raw Milk. Appl. Sci. 2021, 11(23), 11281; (IF = 2.679). https://doi.org/10.3390/app112311281.
Navickaitė D, Ruzgys P., Maciulevičius M, Dijk G., O’Connor R., and Šatkauskas S. Ca2+ roles in electroporation-induced changes of cancer cell physiology: from membrane repair to cell death. Bioelectrochemistry. 2021 Dec;142:107927. (IF = 5.760).
Visockis M., Bobinaitė R., Ruzgys P., Barakauskas J., Markevičius V., Viškelis P., Šatkauskas S. Assessment of plant tissue disintegration degree and its related implications in the pulsed electric field (PEF)–assisted aqueous extraction of betalains from the fresh red beetroot. 2021. Innovative Food Science & Emerging Technologies, 73. (IF = 5,916). https://doi.org/10.1016/j.ifset.2021.102761
Maciulevičius M, Navickaitė D, Chopra S, Jakštys B, Šatkauskas S. Sudden Cell Death Induced by Ca2+ Delivery via Microbubble Cavitation. Biomedicines. 2021 Jan 4;9(1):32. (IF = 5.373). https://doi.org/10.3390/biomedicines9010032
Ruzgys P, Böhringer S, Dokumaci AS, Hari Y, Schürch CM, Brühl F, Schürch S, Szidat S, Riether C, Šatkauskas S, Geiser T, Hradetzky D, Gazdhar A. Electrospray Mediated Localized and Targeted Chemotherapy in a Mouse Model of Lung Cancer. Front Pharmacol. 2021 Apr 20;12:643492. eCollection 2021. (IF = 4.4). https://doi.org/10.3389/fphar.2021.643492
2020
Jakstys B, Jakutaviciute M, Uzdavinyte D, Satkauskiene I, Satkauskas S. Correlation between the loss of intracellular molecules and cell viability after cell electroporation. Bioelectrochemistry. 2020 May 13;135:107550. Online ahead of print. (IF = 5.373).
S Chopra, P Ruzgys, M Maciulevičius, M Jakutavičiūtė, S Šatkauskas. Investigation of plasmid DNA delivery and cell viability dynamics for optimal cell electrotransfection in vitro. Applied Sciences. 2020, 10(17), 6070. (IF = 2.474). https://doi.org/10.3390/app10176070
Gyvyte U, Lukosevicius R, Inciuraite R, Streleckiene G, Gudoityte G, Bekampyte J, Valentini S, Salteniene V, Ruzgys P, Satkauskas S, Zviniene K, Kupcinskas J, Skieceviciene J. The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors. Int J Mol Sci. 2020 Jul 21;21(14):5151. (IF = 4.556). https://doi.org/10.3390/ijms21145151
Novickij V, Balevičiūtė A, Ruzgys P, Šatkauskas S, Novickij J, Zinkevičienė A, Girkontaitė I. Sub-microsecond electrotransfection using new modality of high frequency electroporation. Bioelectrochemistry. 2020 Dec;136:107594. Epub 2020 Jul 3. (IF = 5.760).
Maciulevičius M, Tamošiūnas M, Venslauskas MS, Šatkauskas S. The relation of Bleomycin Delivery Efficiency to Microbubble Sonodestruction and Cavitation Spectral Characteristics. Sci Rep. 2020 May 8;10(1):7743. (IF =4.525). https://doi.org/10.1038/s41598-020-64213-y
Navickaite D, Ruzgys P, Novickij V, Jakutaviciute M, Maciulevicius M, Sinceviciute R, Satkauskas S. Extracellular-Ca2+-Induced Decrease in Small Molecule Electrotransfer Efficiency: Comparison between Microsecond and Nanosecond Electric Pulses. Pharmaceutics. 2020 May 4;12(5):E422. (IF = 4.773). https://doi.org/10.3390/pharmaceutics12050422
Streleckiene G., Inciuraite R., Juzenas S., Salteniene V., Steponaitiene R., Gyvyte U., Kiudelis G., Leja M., Ruzgys P., Satkauskas S., Kupcinskiene E., Franke S., Thon C., Link A., Kupcinskas L, Skieceviciene J. miR-20b and miR-451a Are Involved in Gastric Carcinogenesis Through the PI3K/AKT/mTOR Signaling Pathway: Data From Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model. 2020. Int J Mol Sci, 21 (3) 2020 Jan 29. (IF = 4.183). https://doi.org/10.3390/ijms21030877
Murauskas A., Staigvila G., Girkontaitė I., Zinkevičienė A., Ruzgys P., Šatkauskas S., Novickij J., Novickij V. Predicting Electrotransfer in Ultra-High Frequency Sub-Microsecond Square Wave Electric Fields. Electromagn Biol Med. 2020. 39 (1), 1-8. (IF=1.835).
2019
Novickij V., Čėsna R., Perminaitė E., Zinkevičienė A., Characiejus D., Novickij J., Šatkauskas S., Ruzgys P., Girkontaitė I. Antitumor Response and Immunomodulatory Effects of Sub-Microsecond Irreversible Electroporation and Its Combination With Calcium Electroporation. Cancers (Basel). 2019. 11 (11). (IF = 6.162). https://doi.org/10.3390/cancers11111763
Chopra S, Ruzgys P, Jakutaviciute M, Rimgailaite A, Navickaitė D, Satkauskas S. A Novel Method for Controlled Gene Expression via Combined Bleomycin and Plasmid DNA Electrotransfer. Int J Mol Sci. 2019 Aug 19;20(16). pii: E4047. (IF =4.183). https://doi.org/10.3390/ijms20164047
Ruzgys P, Novickij V, Novickij J, Šatkauskas S. Influence of the electrode material on ROS generation and electroporation efficiency in low and high frequency nanosecond pulse range. Bioelectrochemistry. 2019 Jun;127:87-93. Epub 2019 Feb 6. (IF =5.760) https://doi.org/10.1016/j.bioelechem.2019.02.002
Ruzgys P, Jakutavičiūtė M, Šatkauskienė I, Čepurnienė K, Šatkauskas S. Effect of electroporation medium conductivity on exogenous molecule transfer to cells in vitro. Sci Rep. 2019 Feb 5;9(1):1436. (IF = 4.122). https://doi.org/10.1038/s41598-018-38287-8
2018
Ruzgys P, Jakutaviciute M, Chopra S, Satkauskas S. Enhancement of drug electrotransfer by extracellular plasmid DNA. Arch Biochem Biophys. 2018 Dec 10. pii: S0003-9861(18)30830-0. (IF = 3.118). https://doi.org/10.1016/j.abb.2018.12.010
Ruzgys P, Novickij V, Novickij J, Šatkauskas S. Nanosecond range electric pulse application as a non-viral gene delivery method: proof of concept. Sci Rep. 2018 Oct 19;8(1):15502. (IF = 4.122). https://doi.org/10.1038/s41598-018-33912-y
Baltusnikas J, Satkauskas S, Lundstrom K. Constructing RNA Viruses for Long-Term Transcriptional Gene Silencing. Trends Biotechnol. 2019 Jan;37(1):20-28. Epub 2018 Aug 18. Review. (IF = 13.578). https://doi.org/10.1016/j.tibtech.2018.07.015
Baltusnikas J, Satkauskas S, Lundstrom K. Long-Term Transcriptional Gene Silencing by RNA Viruses. Trends Biochem Sci. 2018 Jun;43(6):397-401. Epub 2018 Apr 11. (IF = 16.63). https://doi.org/10.1016/j.tibs.2018.03.003
Boehringer S, Ruzgys P, Tamò L, Šatkauskas S, Geiser T, Gazdhar A, Hradetzky D. A new electrospray method for targeted gene delivery. Sci Rep. 2018 Mar 5;8(1):4031. (IF = 4.259). https://doi.org/10.1038/s41598-018-22280-2
Novickij V, Ruzgys P, Grainys A, Šatkauskas S. High frequency electroporation efficiency is under control of membrane capacitive charging and voltage potential relaxation. Bioelectrochemistry. 2018 Feb;119:92-97. Epub 2017 Sep 14. (IF =5.760). https://doi.org/10.1016/j.bioelechem.2017.09.006
2017
Rajeckaitė V, Jakštys B, Rafanavičius A, Maciulevičius M, Jakutavičiūtė M, Šatkauskas S. Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation. J Membr Biol. 2018 Feb;251(1):119-130. Epub 2017 Nov 15. (IF =1.696). https://doi.org/10.1007/s00232-017-0005-8
Jakutavičiūtė M, Ruzgys P, Tamošiūnas M, Maciulevičius M, Šatkauskas S. Physical Methods for Drug and Gene Delivery Through the Cell Plasma Membrane. Adv Anat Embryol Cell Biol. 2017: 227:73-92. (IF = 2.163). https://doi.org/10.1007/978-3-319-56895-9_5
Ruzgys P, Tamošiūnas M, Lukinsone V, Šatkauskas S. FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation. J Biomed Opt. 2017 Sep;22(9):1-6. (IF = 2.556). https://doi.org/10.1117/1.JBO.22.9.097001
Kung Y, Lihachev A., Satkauskas S., Wen-Shiang Chen. Electroporation by concentric-type needle electrodes and arrays. Bioelectrochemistry. 2017. 118: 31-37. (IF = 5.760). https://doi.org/10.1016/j.bioelechem.2017.06.014
Gianpiero Pataro G., Bobinaitė R., Bobinas Č., Šatkauskas S., Raudonis R., Visockis M., Ferrari G., Viškelis P. Improving the Extraction of Juice and Anthocyanins from Blueberry Fruits and Their By-products by Application of Pulsed Electric Fields. Food Bioprocess Technol. 2017. (IF=2.576). https://link.springer.com/article/10.1007/s11947-017-1928-x
Jakutavičiūtė M., Ruzgys P, Tamošiūnas M., Maciulevičius M., and Šatkauskas S. Physical drug and gene delivery methods through the cell plasma membrane Advances in Anatomy, Embryology and Cell Biology: Transport Across Natural and Modified Biological Membranes and its Implications in Physiology and Therapy. 2017. Springer. (IF = 2.163).
Meyer L.A.T, Kaselis A., Satkauskas S., Dominique Bagnard D. Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition. Methods in Molecular Biology (Semaphorin Signaling). 2017. 1493: 171-183. (IF =1.17). https://doi.org/10.1007/978-1-4939-6448-2_12
2016
Šatkauskas S. Jakštys B., Ruzgys P., Jakutavičiūtė M. Different Cell Viability Assays Following Electroporation In Vitro. In Handbook of electroporation. Springer. 2016. https://doi.org/10.1007/978-3-319-26779-1_140-1
Kasperiuniene J., Jariwala M., Vaskevicius E., Satkauskas S. Affective Engagement to Virtual and Live Lectures. Information and Software Technologies. 2016. 639: 499-508. https://doi.org/10.1007/978-3-319-46254-7_40
Maciulevičius M, Tamošiūnas M, Jakštys B, Jurkonis R, Venslauskas MS, Šatkauskas S. Investigation of Microbubble Cavitation-Induced Calcein Release from Cells In Vitro. Ultrasound Med Biol. 2016. 42: 2990-3000. (IF =2.298). https://doi.org/10.1016/j.ultrasmedbio.2016.08.005
Kaya M., Akyuz B., Bulut E., Sargin I., Tan G., Erdonmez D., Maheta M., Satkauskas S., Mickevičius S. DNA interaction, antitumor and antimicrobial activities of three-dimensional chitosan ring produced from the body segments of a diplopod. Carbohydrate Polymers. 2016. 146: 80-89. (IF = 4.074). https://doi.org/10.1016/j.carbpol.2016.03.033
Tamošiūnas M, Mir LM, Chen WS, Lihachev A, Venslauskas M, Šatkauskas S. Intracellular Delivery of Bleomycin by Combined Application of Electroporation and Sonoporation in Vitro. J Membr Biol. 2016. 249(5):677-689. (IF = 1.991). https://doi.org/10.1007/s00232-016-9911-4
Tamošiunas M, Kadikis R, Saknite I, Baltušnikas J, Kilikevicius A, Lihachev A, Petrovska R, Jakovels D, Šatkauskas S. Noninvasive optical diagnostics of enhanced green fluorescent protein expression in skeletal muscle for comparison of electroporation and sonoporation efficiencies. J Biomed Opt. 2016. 21:45003. (IF = 2.556). https://doi.org/10.1117/1.jbo.21.4.045003
Lamanauskas N., Pataro G., Bobinas Č., Šatkauskas S., Viškelis P., Bobinaitė R., Ferrari G. Impact of pulsed electric field treatment on juice yield and recovery of bioactive compounds from raspberries and their by-products // Žemdirbystė = Agriculture. 2016. 103: 83-90. (IF = 0.644). https://doi.org/10.13080/z-a.2016.103.011
2015
Maciulevicius M, Tamosiunas M, Jurkonis R, Venslauskas MS, Šatkauskas S. Analysis of metrics for molecular sonotransfer in vitro. Mol Pharm. 2015. 12:3620-7. (IF =4.384). https://doi.org/10.1021/acs.molpharmaceut.5b00347
Jakštys B, Ruzgys P, Tamošiūnas M, Šatkauskas S. Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro. 2015. J Membr Biol. 2015. 248:857-63. (IF =2.457). https://doi.org/10.1007/s00232-015-9813-x
Jurkonis R., Lamanauskas, S., Šatkauskas S. Acoustic estimation of resonance frequency and sonodestruction of SonoVue microbubbles. Archives Acoustics. 2015. 40: 293–300. (IF = 0.816). https://doi.org/10.1515/aoa-2015-0032
Venslauskas MS, Šatkauskas S. Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation. Eur Biophys J. 2015. 44:277-89. (IF = 2.474). https://doi.org/10.1007/s00249-015-1025-x
Sungailaitė S, Ruzgys P, Šatkauskienė I, Čepurnienė K, Šatkauskas S. Efficiency of transmembrane molecular transfer using electroporation in dependence of medium viscosity. J Gene Med. 2015. 17:80-6. (IF = 1.953). https://doi.org/10.1002/jgm.2825
Lamanauskas N., Bobinaitė R., Šatkauskas S. Viškelis P., Pataro G., Ferrari G. Pulsed electric field-assisted juice extraction of frozen/thawed blueberries // Žemdirbystė = Agriculture. 2015. 102: 59-66. (IF = 0.644). https://doi.org/10.13080/z-a.2016.103.011
Bobinaitė R., Pataro G., Lamanauskas N., Šatkauskas S., Viškelis P., and Ferrari G. Application of pulsed electric field in the production of juice and extraction of bioactive compounds from blueberry fruits and their by-products. J Food Sci Technol. 2015. 52:5898-905. (IF = 2.024). https://doi.org/10.1007/s13197-014-1668-0
Lamanauskas N., Šatkauskas S., Bobinaitė R. and Viškelis P. Pulsed Electric Field (PEF) Impact on Actinidia kolomikta Drying Efficiency Journal of Food Process Engineering. 2015. 38: 243–249. (IF = 0.745). https://doi.org/10.1111/jfpe.12161
2014
Kaselis A, Treinys R, Vosyliūtė R, Satkauskas S. DRG Axon Elongation and Growth Cone Collapse Rate Induced by Sema3A are Differently Dependent on NGF Concentration. Cell Mol Neurobiol. 2014. 34: 289-96. (IF =2.29). http://www.ncbi.nlm.nih.gov/pubmed/24338202
Treinys R, Kaselis A, Jover E, Bagnard D, Šatkauskas S. R-Type Calcium Channels Are Crucial for Semaphorin 3A-Induced DRG Axon Growth Cone Collapse. PLoS One. 2014 Jul 17;9(7):e102357. (IF = 3.73). https://doi.org/10.1371/journal.pone.0102357
2013
Lamanauskas N, Novell A, Escoffre JM, Venslauskas M, Satkauskas S, Bouakaz A. Bleomycin delivery into cancer cells in vitro with ultrasound and SonoVue® or BR14® microbubbles. J Drug Target. 2013. 4: 407-414. (IF= 2.696). https://doi.org/10.3109/1061186X.2012.761223
2012
Tamošiūnas M., R. Jurkonis, L.M. Mir, A. Lukoševičius, M.S. Venslauskas, S. Šatkauskas. Microbubble sonodestruction rate as a metric to evaluate sonporation efficiency. Journal of Ultrasound in Medicine. 2012. 12: 1993-2000. (IF =1.245). https://doi.org/10.7863/jum.2012.31.12.1993
Šatkauskas S. Ruzgys P. Venslauskas MS. Towards the Mechanisms for Efficient Gene Transfer into Cells and Tissues by Means of Cell Electroporation. Expert Opinion On Biological Therapy. 2012. 12:275-86. (IF=3.279). https://doi.org/10.1517/14712598.2012.654775
Tamošiūnas M., R. Jurkonis, L.M. Mir, A. Lukoševičius, M.S. Venslauskas, S. Šatkauskas. Adjustment of ultrasound exposure duration to microbubble sonodestruction kinetics for optimal cell sonoporation in vitro. Technology in Cancer research and Treatment. 2012. 11: 375-87. (IF = 1.692). https://doi.org/10.7785/tcrt.2012.500285
2011
Venslauskas M.S., S. Šatkauskas, R. Rodaitė-Riševičienė. Efficacy of the delivery of small charged molecules into cells in vitro. Bioelectrochemistry. 2010. 79: 130-5. (IF = 5.760). https://doi.org/10.1016/j.bioelechem.2009.10.003
Čepurnienė K, Ruzgys P, Treinys R, Šatkauskienė I, Šatkauskas S. Influence of plasmid concentration on DNA electrotransfer in vitro using high-voltage and low-voltage pulses. J. Membr. Biol. 2010. 236: 81-5. (IF=2.189). https://doi.org/10.1007/s00232-010-9270-5
2010
Roth L., E. Koncina, S. Šatkauskas, G. Cremel, D. Aunis and D. Bagnard. The many faces of semaphorins: from development to pathology. Cell. Cellular and Molecular Life Sciences. 2009. 66:649-66. (IF = 5.239). https://doi.org/10.1007/s00018-008-8518-z
2009
Šalomskaitė-Davalgienė S., K. Čepurnienė, S. Šatkauskas, M.S. Venslauskas, L.M. Mir. Extent of cell electrofusion in vitro and in vivo in dependence of cell line. Anticancer Research. 2009. 29: 3125-3130. (IF = 1.414)
Labanauskienė J., V. Kirvelienė, S. Šatkauskas, M. Venslauskas, J. Didžiapetrienė, E. Atkočius. Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro. Medicina. 2009. 45: 372-377. (IF = 0.51). https://doi.org/10.3390/medicina45050047
Gonthier B, Koncina E, Satkauskas S, Perraut M, Roussel G, Aunis D, Kapfhammer JP, Bagnard D. A PKC-dependent recruitment of MMP-2 controls semaphorin-3A growth-promoting effect in cortical dendrites. PloS One 2009;4(4):e5099. Epub 2009 Apr 8. (4.411). https://doi.org/10.1371/journal.pone.0005099
2007
Saulis G., S. Šatkauskas, R. Pranevičiūtė. Determination of cell electroporation from the release of intracellular potassium ions. Analytical Biochemistry. 2007. 360: 273-281. (IF = 3.002). https://doi.org/10.1016/j.ab.2006.10.028
Šatkauskas S. and D. Bagnard. Local Protein Synthesis in Axonal Growth Cones: What Is Next? Cell Adhesion and Migration. 2007. 4: 17-22. (IF = 1.816). https://doi.org/10.4161/cam.1.4.5561
2005
Šatkauskas S., D. Batiuškaitė D., Šalomskaitė-Davalgienė S. and M.S. Venslauskas MS. Effectiveness of tumor electrochemotherapy as a function of electric pulse strength and duration. Bioelectrochemistry. 2005. 65: 105–111. (IF = 5.760). https://doi.org/10.1016/j.bioelechem.2004.08.003
Šatkauskas S., Andre F., Bureau M.F., Mahfoudi A., Scherman D., Miklavčič D. and Mir L.M. The electrophoretic component of the electric pulses determines the efficacy of in vivo DNA electrotransfer. Human Gene Ther. 2005. 16(10): 1194-1201. (IF = 4.793). https://doi.org/10.1089/hum.2005.16.1194
2002
Šatkauskas S., Bureau M.F., Puc M., Mahfoudi A., Scherman D., Miklavčič D. and Mir L.M. Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis. Mol. Ther. 2002. 5: 133-140. (IF = 11.45). https://doi.org/10.1006/mthe.2002.0526
2001
Šatkauskas, S., Bureau, M. F., Mafhoudi, A., Schwartz, B., and Mir L.M. Slow accumulation of plasmid in muscle cells: supporting evidence for a mechanism of DNA uptake by receptor-mediated endocytosis. Mol. Ther. 2001. 4: 317-323. (IF = 11.45). https://doi.org/10.1006/mthe.2001.0465